I understand my problem is not with mojoPortal it self, but I am hoping someone has some ideas on what I can check to make it work. Here are my environments:

Development: Windows 7 Pro (Works)
Testing: Windows XP (Works)
Staging: Windows Server 2003 (Works)
Production: Windows Server 2003 (Does not work)  - https://web.manconinc.com/manconweb2/

mojoPortal Version: 2.3.7.5 MSSQL

The error message I get is: System.IO.FileLoadException: Could not load file or assembly 'AjaxControlToolkit' or one of its dependencies. Strong name signature could not be verified. The assembly may have been tampered with, or it was delay signed but not fully signed with the correct private key. (Exception from HRESULT: 0x80131045)

The root web site is running asp.net 2, and the mojoPortal application is running 4.  The mojoPortal is running in its own pool on both servers.  We (the IT manager and myself) confirmed the file folder does have read & execute / list permissions for both IIS anonymous and ASP.NET. We also checked the ASP.NET Temporary files for permissions. We have run out of things to check.  Any ideas?

Thanks

file permissions can get funky when copying from non domain to domain or when you unzip a zip file on a netwrok share it can also result in weird permission problems. So you may have to re-apply the desired permissions after copying files. You can do it on the top level folder and make it propagate the permission changes down through all the child files and folders.